A liquid chromatograph is a commonly used chromatograph product. It is an instrument that separates the mixture and then analyzes it by using the difference in the distribution ratio between the liquid-solid or immiscible liquid. What is the working principle of the liquid chromatograph? How does the liquid chromatograph work? The system consists of a reservoir, a pump, an injector, a column, a detector, a recorder, and the like. The mobile phase in the reservoir is driven into the system by a high pressure pump. The sample solution enters the mobile phase through the injector and is loaded into the column (stationary phase) by the mobile phase, since each component in the sample solution has two phases. Different partition coefficients, when moving in two phases, after repeated adsorption-desorption distribution processes, the components have a large difference in the moving speed, and are separated into individual components and sequentially flow out from the column. When passing through the detector, the sample concentration is converted into an electrical signal and sent to the recorder. The data is printed in the form of a map. The high performance liquid chromatograph mainly has a sampling system, an infusion system, a separation system, a detection system, and a data processing system. Describe their respective compositions and characteristics. Injection system The injection operation is generally performed using a diaphragm injection injector or a high pressure injection chamber, and the injection volume is constant. This is beneficial for improving the repeatability of the analytical sample. Infusion system The system consists of a high pressure pump, a mobile phase reservoir and a gradiometer. The general pressure of the high pressure pump is 1.47 ~ 4.4X10Pa, the flow rate is adjustable and stable. When the high pressure mobile phase passes through the column, the diffusion effect of the sample in the column can be reduced, and the moving speed in the column can be accelerated. It is advantageous to improve resolution, recover samples, maintain biological activity of the sample, and the like. Mobile phase reservoirs and gradiometers allow the mobile phase to vary with the nature of the stationary phase and sample, including changing the polarity of the eluent, ionic strength, pH, or switching to competitive inhibitors or denaturants. This allows efficient separation of various substances (even if there is only one group difference or isomer). Separation system The system includes columns, connecting tubes, thermostats, and more. The column is generally 10 to 50 cm in length (when two are used together, a connecting tube can be added between the two), and the inner diameter is 2 to 5 mm. It is made of "high-quality stainless steel or thick-walled glass tube or titanium alloy. The housing contains a stationary phase with a particle size of 5-10 μm (consisting of matrix and fixative). The matrix in the stationary phase consists of a resin with high mechanical strength or silica gel, which are inert (such as the basic silicic acid gene on the surface of silica gel). Has been removed), porosity (pore size up to 1000?) and large specific surface area, plus the surface is mechanically coated (as in the preparation of stationary phase in gas chromatography), or chemically coupled to various genes (such as An organic compound of a phosphate group, a quaternary amino group, a methylol group, a phenyl group, an amino group or an alkyl group of various lengths of a carbon chain, or the like. Therefore, such a substance having a fixed relative structure is excellent in selectivity. After pea agglutinin (PSA) is coupled to the surface of the porous silica gel, a glycoprotein in the fibroblast can be isolated. In addition, the fixed phase-based plasmid is small, and the column bed can easily reach a uniform and dense state, and the eddy diffusion effect is easily reduced. The size of the matrix is ​​small, the micropores are shallow, and the sample has a short mass transfer in the microporous region. These are beneficial for reducing the band width and increasing the resolution. According to the theory of column efficiency, the matrix size is small, and the theoretical number N of the tray is larger. This further proves that the small particle size will increase the resolution. In addition, the high-performance liquid chromatograph thermostat can adjust the temperature from room temperature to 60C, and improve the efficiency of the column by improving the mass transfer rate and shortening the analysis time. Shanghai Jiapeng Technology Co., Ltd. specializes in: UV analyzer, three-use UV analyzer, dark box UV analyzer, black box UV analyzer, black box UV analyzer, portable UV analyzer, three-use UV analyzer, dark box , UV detectors, partial collectors, constant current pumps, peristaltic pumps, gel imaging systems, gel imaging analysis systems, chemiluminescence imaging analysis systems, photochemical reactors, vortex mixers, vortex mixers, glass chromatography columns, Gradient mixer, gradient mixer, nucleic acid protein detector, glass chromatography column, fluorescent whitening agent analyzer, fraction collector, rubber cutting instrument, blue gel cutting instrument, chromatography system and other products. Welcome to inquire. Our spa stools including adjustable beauty stool, beauty therapist stool, pedicure stools with footrest, etc. There are several kinds of spa stools for you to choose. You can find the one you want. It is sturdy and durable enough to have a nail spa. We have the perfect after-sales service and technical support. Look forward to your cooperation! Spa Stool,Adjustable Beauty Stool,Beauty Therapist Stool,Pedicure Stools With Footrest TOM SPA BEAUTY SALON EQUIPMENT CO.,LTD , https://www.tomspabeauty.com
What is the working principle of the liquid chromatograph?