In the latest issue (August 31) of "Cell" (Cell) (Cell) (Cell) (Cell) (Cell) (Cell) (United States) published two research papers in animal models confirmed that single-strand short interfering RNA (ss-siRNA) molecules can be widely distributed , Activate RNA interference (RNAi) signals, thereby inhibiting the expression of target genes. RNAi signaling is a mechanism by which living cells suppress gene expression. RNAi signals usually involve the participation of short double-stranded RNAs (dsRNA). The RNAi mechanism removes the "sense strand" of dsRNA, leaving a functional "antisense" single-stranded RNA to bind to the target Mrna, thereby causing degradation. In both studies, scientists and collaborators at Isis Pharmaceuticals confirmed that ss-siRNA molecules can effectively trigger RNAi signals and selectively inhibit the expression of target genes. Free request: High-speed PCR-technical information of the latest series of Jena products >> >> In an article titled "Single-stranded siRNAs activate RNAi in animals," scientists at Isis Pharmaceuticals evaluated the absorption, distribution, metabolism, and activity of ss-siRNAs designed to use RNAi signals. Research data indicates that single-stranded RNA-like antisense oligonucleotides are widely distributed in multiple organs in an active state, and can effectively participate in and trigger RNAi signals without the need for complex formulas or transport carriers that are usually necessary for double-stranded RNAi molecules. Dr. Stanley T. Crooke, Chairman and CEO of Isis Pharmaceuticals, said: "Double-stranded siRNA drugs require complex formulations to achieve adequate delivery to achieve systemic activity. This requirement severely limits the safety and effectiveness of RNAi signaling. Drug development. In contrast, single-stranded RNA-like antisense drugs can be administered subcutaneously and can be distributed to tissues without formulas. In addition, the treatment of RNAi with single-stranded drugs eliminates the need for complementary strands, the 'sense' strands Limit the risk of adverse effects and reduce the cost of manufacturing. At Isis, we have made a major breakthrough in the development of single-stranded RNA-like antisense drugs in oligonucleotide chemistry, through many different antisense mechanisms, including RnaseH , Splicing, and now RNAi to function. " In another paper entitled "Single-stranded RNAs act through RNAi, allele-selectively target expanded CAG repeats, and potently inhibit huntingtin expression", at Isis Corporation and from the University of Texas Southwestern Medical Center and the University of California San Diego The collaborators at the campus designed some ss-siRNA molecules that mimic the activity of microRNA and selectively inhibited the production of a specific mutant, pathogenic HTT protein that is related to the etiology and progression of Huntington's disease. These research data show that ss-siRNAs involved in RNAi signaling selectively silenced the huntingtin gene in animal models of Huntington's disease. Dr. David Corey, a professor of pharmacology at the University of Texas Southwestern Medical Center, said: "In summary, these data provide compelling evidence that single-stranded oligonucleotides can be designed to use RNAi signals to silence specific tissues in target tissues Gene expression of mRNAs. In our initial animal experiments, animals were well tolerated to compounds even at high doses. Single-stranded antisense technology provides a new strategy for using RNAi signals. It combines the potential of RNAi with single-stranded nucleosides The beneficial drug properties of the acid are combined. " Dr. Crooke said: "Together with colleagues at the University of Texas and the University of California, San Diego, we have confirmed that the use of ss-siRNAs can selectively target and specifically silence genes that cause the pathogenic form of huntingtin protein, using RNAi signaling MicroRNA-like effects. We believe that this work lays a good foundation for the development of a powerful drug development platform that uses single-stranded RNA-like antisense drugs to harness RNAi signaling for gene silencing. " Original Index: Single-Stranded siRNAs Activate RNAi in Animals. Cell, Volume 150, Issue 5, 883-894, 31 August 2012 Single-Stranded RNAs Use RNAi to Potently and Allele-Selectively Inhibit Mutant Huntingtin Expression.Cell, Volume 150, Issue 5, 895-908, 31 August 2012 Dog Backpack Carrier,Dog Poop Bags,Dog Carrier Bag,Dog Treat Bag Ningbo Fineweather International Trade Co., Ltd. , https://www.nbfwsbag.com
Two Cell articles focus on new RNA technology