[Question] I would like to ask, when is the one-way immunodiffusion method used, and when is the ELISA method used, why not choose B [Answer] Student gdlj2011, hello! The answers to your questions are as follows: Choose C for the answer to this question. One-way immunodiffusion method: perforate agar plates containing specific antibodies, and add quantitative antigen to the wells. When the antigen diffuses to the surroundings, it will combine with the antibodies in the agar to form a white precipitation ring. The antigen concentration was positively correlated. At the same time, standard antigen or international reference protein is used to make a standard curve, which can be used to quantitatively detect the antigen concentration (mg / ml or IU / ml) of unknown specimens. Using this experimental method, the content and normal value of IgG, IgA and IgM in the serum of normal people or patients can be detected. Enzyme-linked immunosorbent assay (ELISA): It is the most widely used technique in enzyme immunoassay. The basic method is to adsorb the known antigen or antibody on the surface of the solid phase carrier (polystyrene micro-reaction plate) to make the antigen and antibody react on the surface of the solid phase, and wash the antigen-antibody complex on the solid phase with the liquid phase by washing The free components in are separated. Since this method has the advantages of high specificity, strong sensitivity and convenient operation, it has been widely used. There are many kinds of virus detection kits, such as various types of hepatitis virus, herpes simplex virus, cytomegalovirus, Epstein-Barr virus and HIV. (1) Double antibody sandwich method: used to check specific antigens. Coat the known antibody on the enzyme-linked detection plate (solid phase), add the specimen to be tested, if the specimen contains the corresponding antigen, it will bind to the antibody on the solid phase, wash to remove unbound components, and add the antigen-specific enzyme label Antibody, wash away the unbound enzyme labeled antibody, and develop color after adding substrate. Double-antibody sandwich ELISA is also commonly used in the determination of serum IgE by enzyme-linked immunoassay. It is easy to operate and has high sensitivity, and it is often used in clinical practice. (2) Indirect method: used to check specific antibodies. Coat the solid phase with a known antigen, add the serum sample to be tested, add enzyme-labeled secondary antibody, and add substrate to observe the color reaction. So choose C for the answer to this question. Private label skincare face soap ,30 years experience guangzhou cosmetics factory supply ,OEM/ODM service is welcomed . face soap,whitening face soap,papaya face soap,acne face soap Athena(Guangzhou) Cosmetics Manufacturer Co., Ltd , http://www.ydgzathenaskincare.com
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Simple methods commonly used to detect IgE content