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Hepatitis B core antigen (HBcAg) ELISA kit operating method
In the long-term sales practice, Jiehui experiment has formed a unique market advantage: complete products, reasonable prices, stable operation, timely information feedback, and can enjoy the most direct, thoughtful and perfect after-sales service of foreign suppliers anytime, anywhere. Hepatitis B core antigen (HBcAg) enzyme-linked immunoassay kit operating steps: 1. Equilibrium: remove the kit components from the kit and equilibrate to room temperature (18 ℃ -25 ℃). After opening the microplate, Sealed in a ziplock bag. 2. Mixing solution: The concentrated washing solution should be shaken well before preparation (if crystals should be fully dissolved), the concentrated washing solution and distilled or deionized water should be diluted 1:19 before use. 3. Numbering: Fix the micropore strips to the bracket and number them in order. 4. Dilution: Dilute the serum sample to be tested 1: 1000. 5. Add sample: add 100μl of the diluted test serum sample or negative and positive control serum to the corresponding wells in order. 6. Incubation. Incubate at 37 ° C for 20 minutes. 7. Washing: Wash thoroughly 5 times with washing solution, and buckle to dry after washing (soak time should be maintained for 30-60 seconds each time). 8. Add antigen and enzyme: add 50μl of antigen (HBcAg) and enzyme-labeled antibody to each well; mix gently by tapping. 9. Incubation: Incubate at 37 ° C for 40 minutes. 10. Washing: Wash thoroughly 5 times with washing solution, and buckle to dry after washing (each time should maintain 30-60 seconds soaking time). 11. Color development: add 50μl of substrate A and B to each well, mix gently by tapping, and leave in the dark for 15 minutes at room temperature. 12. Termination: Add 50μl of stop solution to each well and mix well. 13. Measurement: use a microplate reader to measure the OD value of each well at a single wavelength of 450 nm or a dual wavelength of 450 nm / 630 nm (when using a single wavelength for measurement, a blank control should be set, and the measurement should be completed within 30 minutes, and the results should be recorded) Cautions for HBcAg ELISA kit: 1. For serum dilution, please use 10mmol / LPBS or normal saline. It should not be diluted with distilled water. 2. It is recommended to set two wells for negative and positive control sera for each plate. When setting blank control, no samples, antigens and enzyme-labeled antibodies are added, and the rest of the steps are the same. 3. Each well must be filled up during washing to prevent free enzymes from being washed out. 4. Turn over the reagent bottle several times before adding the reagent to mix the liquid. If dropping, 1-2 drops should be discarded before dropping. When dropping, the bottle body should be kept vertical to make the drop volume accurate. Be careful not to drip reagent on the wall of the well. 5. All samples should be handled according to the source of infection. 6. Instructions for use of parafilm. (1) After unpacking the microplate, after taking out the microwell strips required for the day, the remaining microwell strips can be sealed with parafilm to avoid moisture. When sealing, be careful not to stick the parafilm to the bottom of the microporous strip, so as not to affect its light transmittance. (2) When incubating the microplate, cover the orifice with parafilm to avoid the unintended impact of other factors on the experiment. 7. Reagents with different product names and different batch numbers should not be mixed to avoid erroneous results. 8. The use of EDTA, sodium citrate and heparin sodium as anticoagulants does not affect the experimental results. Teachers are welcome to order the "Hepatitis B Core Antigen (HBcAg) ELISA Kit". We will serve you wholeheartedly, so that you can buy it with confidence, satisfaction and comfort.