The application of ultraviolet analyzer to fluorescence technology, the application of ultraviolet analyzer in biochemistry and molecular biology research mainly includes the following aspects: 1. Qualitative of substances: Different fluorescent substances have different excitation and emission spectra, so ultraviolet analyzers can be used to identify substances. Compared with absorption spectroscopy, Fluorescence method has higher selectivity. 2. Quantitative determination: The ultraviolet analyzer can quantitatively analyze the content of the fluorescent component in the sample by using the relationship between the fluorescence intensity and the sample concentration at a lower concentration, which is commonly used for determination The content of amino acids, proteins, nucleic acids. One of the advantages of fluorescence quantitative measurement is its high sensitivity. For example, the determination limit of vitamin B2 can reach 1 ng / ml. This advantage The amount of sample required for the measurement is greatly reduced. 3. This quantitative determination method of ultraviolet analyzer can also be applied to enzyme-catalyzed reactions. As long as there is a change in fluorescence intensity before and after the reaction, it can be used to determine the enzyme content and the rate of enzyme reaction. Let's understand what is fluorescence and fluorescence technology. Fluorescence refers to a luminescence phenomenon of photoluminescence. When a certain temperature substance passes the incident light of a certain wavelength (Usually ultraviolet or X-ray) irradiation, enter the excited state after absorbing light energy, and immediately de-excite and emit outgoing light with a longer wavelength than the incident light (Usually the wavelength is in the visible light band); and once the incident light is stopped, the luminescence phenomenon also disappears immediately. The outgoing light with this property is called fluorescence. The application of this luminescence phenomenon in various aspects and related methods are called fluorescent techniques.
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Specific application of UV analyzer