Liquid-based thin-layer cytology (TCT) technology and microscope realization method

What is liquid-based thin-layer cytology (TCT)?

In recent years, a modern cervical cytology smear technology, namely liquid-based thin-layer cytology (TCT) has been more and more widely used in cervical cytology screening. The TCT test uses a liquid-based thin-layer cell detection system to detect cervical cells and carry out the internationally accepted TBS cytology classification diagnosis. It is currently the most advanced cervical cancer research technology in the world, which is different from the traditional cervical scraping Pap smear. Compared with the test, it significantly improves the satisfaction of the specimen and the detection rate of cervical abnormal cells. The detection rate of cervical cancer cells by TCT test is close to 100%. At the same time, it can also find precancerous lesions, microbial infections such as mold, trichomonas, and chlamydia. Wait. At present, TCT technology has been applied to the screening of women's cervical cancer in large and medium cities in Europe, America and China. Tan Xianjie, Department of Gynecology, Peking Union Medical College Hospital

Because the traditional smear reading method will have a false negative rate of 2% to 50%, except for the fatigue of the human eye and the coated cells are not at a level that affects the diagnosis, there are a large number of red blood cells, white blood cells, mucus and shedding on the smear Necrotic tissues etc. affect the correct diagnosis. In order to solve the latter problem, cell engineering experts have introduced a new technology called liquid-based thin-layer cytology in recent years, that is, the impurities on the smear are removed by technical processing, and the thin-layer smear is clearly made for observation, so that The filmmakers are easier to observe, and their diagnostic accuracy is higher than traditional methods. At present, there are two kinds of imported equipment in China: (1) Thin layer cytologic test system (Thinprep cytologic test, TCT): It was approved by the US FDA for clinical use in 1996. The main method is to wash the cervix exfoliated cells into the vial containing the cell preservation solution, the scraper brush is stirred in the vial for tens of banknotes, and then filtered through a high-precision filter membrane, the impurities in the specimen are separated and filtered The epithelial cells were made into thin layer cells with a diameter of 20mm on a glass slide, fixed with 95% alcohol, pasteurized and mounted, and examined by a cytology expert under the microscope, and a diagnosis report was made according to the TBS method. This method improves the diagnosis rate of abnormal cells by 13% and the detection rate of low-grade squamous epithelial lesions by 65%, but the device can only process one specimen at a time. (2) Autocyto prep cytologic test (Liquid-based cytologic test, LCT): It was approved by the US FDA for clinical use in 1999. The basic method is to centrifuge the collected cell preservation solution and separate the mucus, blood and inflammatory cells in the specimen by natural precipitation method, and collect the remaining epithelial cells to make ultrathin cells with a diameter of 13mm on the glass a. Each time, 48 specimens can be processed at the same time, and the cells can be stained at the same time in the fully automatic production process to achieve higher quality and higher efficiency. This technology reduces the reading range to 13mm in diameter (and the former is 384mm2

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