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Users have two basic requirements for the incubator. One is that the incubator can provide the most accurate and stable control of temperature, humidity and carbon dioxide concentration, so as to facilitate the progress of its research work; the other is that the incubator can The microbial contamination in the box is effectively prevented, and the pollution can be eliminated regularly to protect the research results and prevent sample loss.
1. Temperature control
1. Heating method:
Both gas-jacketed heating and water-jacketed heating are accurate and reliable, and they have their own advantages and disadvantages. Water jacket heating is to maintain a constant temperature by surrounding the inner box with an independent water jacket layer. Its advantages: water is a good thermal insulation substance. When a power failure occurs, the water jacket system can Maintain the temperature accuracy and stability in the incubator for a long time (the time to maintain the temperature constant is 3-4 times that of the gas-jacketed system), which is conducive to the unstable experimental environment (such as the limitation of useful electricity or frequent power outages) Of users. Air-jacket heating directly heats the inner box through heaters located in the gas jacket layer of the box, also known as six-sided direct heating. Compared with the water-jacket type, the air-jacket type has the characteristics of faster heating and faster temperature recovery than the water-jacket type incubator, which is especially beneficial for cultivation that requires frequent opening and closing of the door. In addition, for the user, the air jacket design is simpler than the water jacket type (the water jacket type needs to add water, empty and clean the water tank, and constantly monitor the operation of the water tank).
2. Temperature control system:
Maintaining a constant temperature in the incubator is an important factor for maintaining healthy growth of cells, so an accurate and reliable temperature control system is an indispensable important part of the incubator. The user selects the incubator with independent triple temperature control function, that is, the main heating unit to provide accurate temperature control; the bottom heating unit, heating the water tray to control humidity; our heating unit, to prevent condensation water from the glass observation door, and help you open after opening The temperature is restored, thus ensuring the stable operation of the incubator.
3. Temperature uniformity:
The temperature uniformity in the incubator box is also the main factor that users need to consider. Generally, the uniformity of the incubator equipped with fans and air ducts in the box is much better. At the same time, this device also helps the temperature and relative humidity in the box quickly restore.
2. Control of carbon dioxide concentration:
At present, the control method of carbon dioxide concentration in the cabinets on the market is mainly measured by infrared sensors (IR) or thermal conductivity sensors (TC D), and the carbon dioxide concentration in the cabinet is monitored by the adjustment of the built-in microcomputer. Both sensors are accurate, but each has advantages and disadvantages. The working principle of the thermal conductivity sensor to monitor the concentration of CO2 is based on the continuous measurement of the air thermal conductivity of the inner cavity. The low thermal conductivity of the input CO2 gas will change the thermal conductivity of the air in the cavity, which will produce a direct Proportional electrical signal. Infrared sensor (IR) It uses an optical sensor to detect CO2 levels. The IR system includes an infrared emitter and a sensor. When the CO2 in the box absorbs part of the infrared light emitted by the transmitter, the sensor can detect the amount of infrared reduction, and the amount of infrared light absorbed corresponds to the level of CO2 in the box. , So that the concentration of CO2 in the box can be obtained. Because the IR system determines the CO2 concentration in the box through the reduction of infrared rays, and the particulate matter in the box can reflect or partially absorb infrared rays, making the IR system more sensitive to the amount of particulate matter in the box, so the IR sensor is applied to the incubator with high-efficiency air filter It is more appropriate.
3. Relative humidity:
Humidity in the box is a very important factor for cultivation, but it is often overlooked. Maintain a sufficient humidity level and a fast enough humidity recovery rate (such as after opening and closing the door) to ensure that the culture will not fail due to excessive drying. When choosing an incubator, the user should try to choose the one with fast evaporating humidity. It is better to have a drainage duct to better ensure that the cabinet reaches the maximum relative humidity quickly and the humidity recovery time after opening and closing the door is fast.
4. Anti-pollution design and disinfection and sterilization system:
Contamination is a major factor that leads to cell culture failure. Therefore, incubator manufacturers have designed a variety of different devices to reduce and prevent the occurrence of pollution. The main way is to minimize the areas and surfaces where microorganisms can grow, and Combined with an automatic decontamination device to effectively prevent pollution. For example, the incubator with high-temperature humid heat sterilization, UV sterilization, dry heat sterilization and other functions can realize the sterilization of the incubator after accidental contamination; if there is a ULPA filter designed and produced inside the box, it can filter the incubator The internal air can be filtered to remove 99.999% of particles above 0.12 microns; in addition, if the cabinet has an antibacterial coating on the outside, it can effectively keep microorganisms growing on the surface of the cabinet. These devices are essential for cell culture, so what kind of disinfection method is more suitable for the incubator? First of all, what we consider is of course the sterilization ability of various methods. The ultraviolet sterilization ability is inversely proportional to the square of the distance of the ultraviolet lamp from the target. The farther the distance, the worse the sterilization ability, so the ultraviolet sterilization method has its limitations It is difficult to meet the requirements of complete sterilization; in comparison, high temperature disinfection is currently a more effective method of disinfection and sterilization. High temperature disinfection is divided into two categories, one is traditional high temperature dry heat disinfection, and the other is advanced high temperature Damp heat sterilization. Next, we focus on the pros and cons of high-temperature dry heat and high-temperature humid heat. High temperature and humid heat have large latent heat of steam and strong penetration, which is easy to denature or solidify the protein. Therefore, the sterilization efficiency of this method is higher than that of dry heat sterilization. There are three reasons: â‘ The temperature required for protein solidification is related to its water content. The larger the water content, the lower the temperature required for solidification to occur. The moist heat-sterilized bacterial protein absorbs water, so it is easier to solidify in dry hot air at the same temperature. â‘¡ During the process of humid heat sterilization, steam releases a lot of latent heat to accelerate the increase of humidity. Therefore, the temperature required for wet heat sterilization is lower than that for dry heat. For example, at the same temperature, the time required for wet heat sterilization is shorter than that for dry heat. â‘¢ The penetration of damp heat is greater than that of dry heat, so that the deep part can reach the sterilization temperature, so damp heat is better than dry heat. Therefore, high-temperature sterilization is not simply looking at the sterilization temperature, mainly to see whether it is humid heat sterilization.
5. Other factors The volume of the incubator is also a factor that cannot be ignored. It is not enough to buy a small one, and it is wasteful and takes up space when it is large. At this time, you need to have a better understanding of the range of the required incubator volume before purchasing, and reserve a little more space on this basis to ensure that it is needed from time to time. In addition, the upgrade function of the incubator is also very important. It is best to choose an incubator that can be upgraded with oxygen. In the future, it can be easily upgraded to achieve the function of a three-gas incubator. In addition, every user hopes that the equipment used can be convenient Easy to use, the micro-processing control system and other various functional accessories are also important, such as intelligent data and event detectors, which can record all operating parameters during the use of the incubator, and can be transferred to the record on the LCD display through the program software The data, such as the built-in flash memory, can realize the long-term storage of operating data. If it has a diagnostic interface and online quick help function, it can provide a comprehensive solution to the problems often encountered during use, further ensuring the operation and incubation of the incubator. The control is very simple.
Precautions for the purchase of carbon dioxide incubator
In today's era, everyone knows that carbon dioxide incubators are widely used in life science cell culture research. Typical applications include medicine, immunology, microbiology, genetics, agricultural science, and pharmaceutical research and production, and have become the most commonly used in laboratories. It is one of the conventional instruments, which simulates a growth environment like cells / tissues in the organism such as constant pH (pH value: 7.2-7.4), stable temperature (37 ° C), high Relative humidity (95%), a device for in vitro culture of cells / tissues.
The above is only the simple information provided by Shanghai Bilang Instrument Co., Ltd. for the purchase of carbon dioxide incubators. If you are interested in this product or need this product, you can inquire / 021-52965969, Shanghai Bilang Instrument Co., Ltd. looks forward to your Call and serve you wholeheartedly!