Overall Reflex LED Operating Light
Germany imported beads
Imported French lens
mould Die-casting Eight edge type Revolving arm
Optional emergency power supply≥3 hours
Main Feature:
1.ZF series of reflective shadowless lamp is widely used in various occasions operation lighting needs,is the ideal lighting equipment of modern operation room.
2.Full close streamline lamp body design completely meets the demand of the sterilization and high standard of laminar flow purification in operation room.
3.Adopting more than 5280 slices of reflection mirror, guaranteeing 1200 mm lighting depth.
4.Special design of color temperature compensation provides closer to that of natural sunlight and helps the doctor to distribute operation parts clearly and accurately.
5.The back-up bulb will start work within 0.2 seconds automatically in case the main bulb is damaged,ensuring the continuation of surgical operation. There`s failure indication in the handle control panel,to recommend the bulb to be changed after operation.
6.Detachable handle jacket ,can be used for high temperature (≤134℃)sterilized.
7.Digital-control circuit offers many functions, with 10 segment brightness selection stepless lighting regulation,brightness memory, low voltage start-up and power on self test etc.
8.The life-span of OSRAM halogen bulb is up to 1500 hours; the bulbs can be changed easily.
9.Germany ORSAM lamp socket,thermostability
10.Balance arm can be optional Germany imported or domestic.
11.Mould Die-casting Eight edge type Revolving arm
Overall Reflex LED Operating Light,LED Surgical Lights,Overall Reflex LED Operating Light,Reflection Operating LED Light Shandong qufu healthyou Medical Technology co.,Ltd , https://www.kangerjianmedical.com
Monkey rabies virus antibody (PRV-Ab) ELISA test kit
This kit can only be used for scientific research, and should not be used for medical diagnosis of monkey rabies virus antibody (PRV-Ab) ELISA test kit. Instructions for use The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with the monkey rabies virus antigen, the specimen, the standard, and the HRP-labeled detection antibody were sequentially added, and the cells were washed and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the monkey rabies virus antibody (PRV-Ab) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration. Sample Collection, Handling and Preservation Method 1. Serum: Use a tube containing no pyrogen and endotoxin to avoid any cell irritation during the operation. After collecting the blood, centrifuge and centrifuge for 10 minutes at 3000 rpm to quickly and carefully separate the serum and red blood cells. 2. Plasma: EDTA, citrate or heparin anticoagulation. The supernatant was taken by centrifugation at 3000 rpm for 30 minutes. 3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymer. 4. Tissue homogenization: The tissue is added to the appropriate amount of physiological saline and chopped. The supernatant was taken by centrifugation at 3000 rpm for 10 minutes. 5. Storage: If the sample is not detected in time after collection, please dispense it once, freeze it at -20 °C, avoid repeated freezing and thawing, thaw at room temperature and ensure that the sample is fully thawed evenly. Self-prepared items 1. Microplate reader (450nm) 2. High-precision sampler and tip: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL3. 37°C incubator operation precautions 1. Kit storage At 2-8 ° C, equilibrate for 20 minutes at room temperature before use. The concentrated washing liquid taken out from the refrigerator will crystallize, which is a normal phenomenon, and the water bath is heated to completely dissolve the crystals before use. 2. The slats not used in the experiment should be immediately put back into the ziplock bag and sealed (low temperature dry) for storage. 3. The pre-treated sample does not need to be diluted, and 50 μL of the sample can be directly added. 4. Incubate the operation strictly in accordance with the time indicated in the instructions, the amount of liquid added, and the sequence. 5. Shake well all liquid components before use. Kit Composition Name 96-well configuration 48-well configuration Remarks Microporous microplates 12 wells × 8 strips 12 wells × 4 no standard (6 tubes) 0.5 ml / tube 0.5 mL / tube without sample dilution 6 mL 3 mL No detection antibody -HRP 10mL 5mL No 20× Wash Buffer 25mL 15mL Diluted substrate A 6mL 3mL without substrate B 6mL 3mL without stop solution 6mL 3mL without sealing film 2 sheets 2 sheets without instructions 1 part 1 without zipper bag 1 1 no note: 1. The concentration of the standard is: 8, 4, 2, 1, 0.5, 0.25 ng/mL. 2. If the sample value exceeds the highest concentration of the standard, the sample may be diluted appropriately with the sample diluent. . Preparation of reagents 20× Wash buffer dilution: Distilled water was diluted 1:20, ie 1 part of 20× wash buffer plus 19 parts of distilled water. Washing method 1. Manually wash the plate: Drain the liquid in the hole, fill each hole with the washing liquid, let stand for 1 min, then drain the liquid in the hole, pat dry on the absorbent paper, and wash the plate 5 times. 2. Automatic washing machine: Inject 350μL of washing solution into each hole, soak for 1min, and wash the plate 5 times. Procedure 1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats were sealed back to 4 °C with a ziplock bag. 2. Set standard hole, sample hole and blank hole, no blank hole is added; standard product hole is added with different concentration of standard product 50μL; 3. Sample hole to be tested is added with 50μL sample to be tested; 4. Subsequent standard 100 μL of horseradish peroxidase (HRP)-labeled detection antibody was added to the wells and sample wells (no blank cells were added), and the reaction wells were sealed with a sealing plate and incubated for 60 min in a 37 ° C water bath or incubator. 5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine). 6. Add 50 μL of substrate A and B to all wells and incubate for 15 min at 37 ° C in the dark. 7. Add 50 μL of stop solution to all wells and measure the OD value of each well at a wavelength of 450 nm within 15 min. The result is judged to draw a standard curve: in the Excel worksheet, the standard product concentration is used as the abscissa, the corresponding OD value is plotted as the ordinate, the standard linear regression curve is drawn, and the sample concentration values ​​are calculated according to the curve equation. Kit Performance 1. Accuracy: The linear regression and standard concentration correlation coefficient R value of the standard is greater than or equal to 0.9900. 2. Sensitivity: The minimum detection concentration is less than 0.1 ng/mL. 3. Specificity: Does not cross-react with other soluble structural analogs. 4. Repeatability: The coefficient of variation between the plates and the plates is less than 15%. 5. Storage: 2-8 ° C, protected from light and moisture. 6. Validity: 6 months Disclaimer 1. The kit is for research use only and should not be used for clinical trials or human experiments. Otherwise, all consequences will be borne by the experimenter and the company will not be responsible. 2. Operate in strict accordance with the instructions, the experimenter violates the instructions, and the consequences are borne by the experimenter.