Schisandra, known as Schisandra chinensis, is the fruit of Schisandra chinensis (Turcz.) Baill., a plant of the genus Schisandra chinensis (Turcz.) Baill. It has the effects of astringent, qi, qi and spleen. The study found that Schisandra chinensis contains lignans, organic acids, volatile oils, polysaccharides and other ingredients. Among them, Schisandra chinensis polysaccharide has pharmacological effects such as liver protection, anti-fatigue, immune promotion, anti-oxidation, anti-aging and anti-tumor, and has good clinical efficacy. Since the quality of polysaccharide is an important prerequisite for ensuring the clinical efficacy of Schisandra, the quality of polysaccharides in Schisandra, especially the accuracy of its mass fraction determination, has higher requirements. At present, the methods for detecting polysaccharides in Schisandra chinensis are mainly high-performance liquid chromatography and colorimetry. The former is less used because of the high-purity schisandra polysaccharide reference substance, and the latter is used as a classical method for measuring polysaccharides. Easy to work, the results are accurate and reliable, and have been widely used in the research of Schisandra polysaccharides. Shanghai Chuangsai provides schisandrol, 69363-14-0, HPLC≥98%, C78-A0823-20mg, and the price is 640 yuan. At present, the colorimetric method for measuring the polysaccharide of Schisandra chinensis is mainly the phenol sulfate method, the sulfonate method, and the 3,5-dinitrosalicylic acid method (DNS). The glucose is used as the reference substance to calculate the quality of the polysaccharide in the sample. fraction. The phenolic sulphate method and the sulphate sulphate method are obtained by hydrolyzing a polysaccharide by sulfuric acid, dehydrating to form furfural or a derivative thereof, and maximally absorbing at a specific wavelength after coloring with a corresponding color developing agent, and the principle of color development by a phenolic acid method and an anthranyl sulfate method. In the same manner, the polysaccharide is hydrolyzed to a monosaccharide under strong acidic conditions, and the monosaccharide is further dehydrated to form an aldehyde derivative to develop color. However, the results of this experiment show that the results of the two methods for the determination of polysaccharides in Schisandra chinensis differ by nearly 10%, probably because the different monosaccharides constituting the component differ from the phenol sulfate or sulfonium ketone reagent in terms of reaction conditions, resulting in differences in results. . The optimal reaction conditions for the composition of Schisandra monosaccharide and the two color development methods are for further study. Shanghai Chuangsai provides ELISA kit 96T/48T, rat nuclear factor κB receptor activating factor ligand Elisa kit, LH-E10218RA-96T, the price is 1700 yuan. The DNS method is to produce a brown-red compound by co-heating with a reducing reagent by a DNS reagent, and has a maximum absorption at a specific wavelength, and then determining the mass fraction of the monosaccharide in the total sugar test solution and the monosaccharide test solution, and calculating the The monosaccharide mass fraction of the sugar test solution is subtracted from the monosaccharide mass fraction of the total sugar test solution, and finally the polysaccharide mass fraction is calculated. When the mass fraction of monosaccharides in the monosaccharide test solution was determined by the DNS method, it was found that the absorption value was extremely small and almost negligible. This result indicates that the mass fraction of monosaccharide in Schisandra chinensis polysaccharide extract is lower, which may be caused by refluxing with 80% ethanol before water extracting Schisandra polysaccharide, removing impurities such as lignans and monosaccharides, and eliminating their determination of polysaccharide mass fraction. Impact, so the advantage of using the DNS method is not obvious. From the analysis and comparison of various aspects of the data, it is found that the phenol method is relatively stable and easy to operate. Therefore, this method is considered to be an ideal method for the determination of polysaccharides in Schisandra. However, since phenol is easily oxidized, it is gradually oxidized to a reddish substance when exposed to light or oxygen, so it should be protected from light in the measurement and the operation should be rapid. The quality of CANSPEC provides excellent performance, and its excellent quality meets the various needs of different experiments. Spot, biochemical reagents, APIs, pharmaceutical intermediates, scientific instruments, laboratory consumables, Shanghai Chuangsai, mass products special promotions, welcome Call us! China Black Cotton Tote Bag,Cotton String Bags supplier & manufacturer, offer low price, high quality Blank Cotton Tote Bags,Cheap Canvas Totes, etc. Black Cotton Tote Bag,Cotton String Bags,Blank Cotton Tote Bags,Cheap Canvas Totes Suzhou Yuli Industrial Co.,Ltd , https://www.packageyuli.com
Method for determination of polysaccharides in Schisandra