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Instruction manual of H. pylori antigen rapid test strip
Instruction manual of H. pylori antigen rapid test strip
Test principle
.HP antigen rapid test strip should use immunochromatography to legally detect H. pylori in stool samples. The reaction membrane in the detection zone is pre-coated with polyclonal antibody to bind .HP antigen.
During the detection process, the sample can be reacted with the colored conjugate (anti-HP polyclonal antibody-red polystyrene microsphere) pre-stained in the test strip to obtain the conjugate. The conjugate then continues to move forward on the membrane by capillary action. As the sample flows through the detection membrane, the colored particles move. When there is a positive result, the specific antibody on the membrane will capture the colored conjugate. The conjugate continues to move forward to the antibody position where the quality control band is fixed, generating a green band. The appearance of the green band has two meanings: 1) confirm that a sufficient amount of sample is added; 2) confirm that the flow direction is correct; 3) act as an internal quality control of the reagent.
Storage and stability
Sealed and stored at 2-30ºC, the test strip can be stabilized to the shelf life of each package. The test strip is not opened until it is used. Do not freeze.
Precautions
1. In vitro diagnosis for professionals only
2. Do not use expired products
3. All samples need to consider the potential harmfulness and be handled according to the pollutant treatment method
4. After the test, discard the discarded test card in an appropriate biohazard container.
Specimen collection and preparation
Fecal specimens should be stored in clean containers and tested immediately after sampling. Samples can be stored in cold storage (2-4 ℃) for 1-2 days, frozen at -20 ℃ for up to 1 year. If frozen, it should be completely thawed and returned to room temperature before testing.
Sample preparation (see picture below)
(1) Unscrew the cap and pick a little specimen with a small stick. If the fecal specimen is liquid, draw 100ul with a suction gun and put the removed sample into a test tube.
(2) Mix the stool sample with the diluent, and shake the test tube to fully disperse the sample.